Open AccessOpening of Ca2+-Dependent K+ Channels by Nordihydroguaiaretic Acid in Porcine Coronary Arterial Smooth Muscle Cells
Author(s) -
Norihiro Nagano,
Yuji Imaizumi,
Makoto Hirano,
Minoru Watanabe
Publication year - 1996
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.70.281
Subject(s) - nordihydroguaiaretic acid , medicine , cardiology , chemistry , biophysics , biology , biochemistry , lipoxygenase , enzyme
Effects of nordihydroguaiaretic acid (NDGA) on the Ca(2+)-dependent K+ channel (BK channel) were examined by the patch clamp technique in single smooth muscle cells of porcine coronary artery. The open probability of BK channels in inside-out patches increased by about 30 times at the holding potential of 0 mV, when 10 microM NDGA was added to the bathing solution (pCa 7.0). The effect of NDGA was concentration-dependent in the range of 1-100 microM and partly removed by washout. The enhancement of BK channels by NDGA was not observed when the cytosolic Ca2+ concentration was very low (pCa > 8.5). These results clearly indicate that NDGA possesses a BK channel opening property in coronary arterial myocytes.