Open AccessActeoside, a Component of Stachys Sieboldii MIQ, May Be a Promising Antinephritic Agent (3): Effect of Acteoside on Expression of Intercellular Adhesion Molecule-1 in Experimental Nephritic Glomeruli in Rats and Cultured Endothelial Cells
Author(s) -
Kazutaka Hayashi,
Tadashi Nagamatsu,
Mikio Ito,
Hideo Yagita,
Yoshio Suzuki
Publication year - 1996
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.70.157
Subject(s) - chemistry , intercellular adhesion molecule 1 , umbilical vein , cell adhesion molecule , intracellular , in vitro , in vivo , microbiology and biotechnology , adhesion , pharmacology , inflammation , immunology , biochemistry , biology , organic chemistry
It is known that adhesion molecules play a crucial role in the development of glomerulonephritis. Therefore, we investigated the effects of acteoside on the expression of intercellular adhesion molecule-1 (ICAM-1) in nephritic glomeruli, in vivo, and human umbilical vein endothelial cells (HUVECs) and rat mesangial cells, in vitro. Aceteoside treatment significantly decreased the up-regulation of ICAM-1 expression in nephritic glomeruli. Acteoside prevented the up-regulation of ICAM-1 expression mediated by inflammatory cytokines or phorbol 12-myristate 13-acetate on HUVECs and rat mesangial cells. Adhesion of neutrophils and macrophages to acteoside-treated HUVECs was suppressed to one half of that in untreated HUVECs. These data support the finding that acteoside inhibits the up-regulation of ICAM-1 in the nephritic glomeruli. Additionally, it is suggested that the antinephritic action of acteoside is due to the inhibition of intraglomerular accumulation of leukocytes through the prevention of the up-regulation of ICAM-1. This is the first paper demonstrating that the up-regulation of ICAM-1 in nephritic glomeruli is inhibited by a natural product, acteoside.