Open AccessInhibitory Effect of KBT-3022, a New Anti-platelet Agent, on Infiltration of Polymorphonuclear Leukocytes Induced by Leukotriene B4 or Formyl-Methionyl-Leucyl-Phenylalanine in Mice
Author(s) -
Koichi Yokota,
Norio Yamamoto,
Yuji Obata,
M. Oda
Publication year - 1995
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.68.353
Subject(s) - leukotriene b4 , polymorphonuclear leukocyte , platelet , chemistry , inhibitory postsynaptic potential , granulocyte , infiltration (hvac) , phenylalanine , pharmacology , immunology , biochemistry , medicine , inflammation , in vitro , amino acid , materials science , composite material
We devised a method for evaluating polymorphonuclear leukocyte (PMN) infiltration in vivo employing an air bleb technique combined with measurement of myeloperoxidase (MPO) activity, and the effects of some anti-platelet agents were evaluated. KBT-3022 (ethyl 2-[4,5-bis(4-methoxyphenyl)thiazol-2-yl]pyrrol-1-ylacetate) and cilostazol inhibited the increase in MPO activity in the connective tissue around the air bleb induced by leukotriene B4 (LTB4) and formyl-methionyl-leucyl-phenylalanine (fMLP). Indomethacin inhibited only the fMLP-induced increase in MPO activity, but ticlopidine hydrochloride and acetylsalicylic acid had no effect. Histologic observation confirmed the inhibition of PMN infiltration by KBT-3022. These results indicate that KBT-3022 may be a potent inhibitor of both LTB4- and fMLP-induced infiltration of PMNs.