Open AccessChanges in 3H-Quinuclidinyl Benzilate Binding and Protein Synthesis in the Striatum Following Chronic Administrations of Muscarinic Agonist
Author(s) -
Kazuo Honda,
Yukio Takáno,
Hiro-o Kamiya
Publication year - 1995
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.67.83
Subject(s) - oxotremorine , muscarinic acetylcholine receptor , quinuclidinyl benzilate , striatum , chemistry , agonist , medicine , endocrinology , muscarinic agonist , receptor , biochemistry , biology , dopamine
Injection of the muscarinic agonist oxotremorine into rat striatum for seven consecutive days resulted in a 44% reduction in 3H-quinuclidinyl benzilate (3H-QNB) binding, and it caused decreases in binding affinity to agonists. Furthermore, it eliminated the shift from the high affinity site to the low affinity site that occurs in the presence of 5'-guanylyl imidodiphosphate (Gpp (NH)p). In addition, oxotremorine caused a 1.5-fold increase in the incorporation of 3H-leucine into the striatum, suggesting that it increased the syntheses of proteins other than the muscarinic receptor protein. The present results show that chronic treatment of the striatum with oxotremorine causes alterations in not only the quantity, but also the sensitivity of muscarinic receptors to guanine nucleotide.