Open AccessEffects of Serotonin1A Agonists on Anoxia-Induced Impairment of Protein Synthesis in Rat Brain Slices
Author(s) -
Makoto Suzuki,
Takehisa Matsuda,
Pranee Somboonthum,
Shoichi Asano,
Kazuhiro Takuma,
Hiroyuki Nogi,
Akemichi Baba
Publication year - 1995
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.67.403
Subject(s) - in vivo , neuroprotection , chemistry , incubation , in vitro , ipsapirone , ischemia , pharmacology , biochemistry , buspirone , biology , medicine , serotonin , receptor , microbiology and biotechnology
Earlier in vivo experiments suggest that serotonin1A (5-HT1A) agonists are new tools for the treatment of experimental cerebral ischemia. The present study examined this idea in an in vitro system. Incubation of rat brain slices under anoxic conditions for 30 min decreased protein synthesis that was assayed in a normoxic medium by measuring the incorporation of [14C]lysine into trichloroacetic acid-insoluble tissue extracts. The 5-HT1A agonists 8-hydroxy-2-(di-n-propylamino) tetralin (10-100 microM) and buspirone (50 microM) attenuated the anoxia-induced decrease in protein synthesis in the slices. Although the degree of the effect is small, it may be relevant to the neuroprotective effect in the in vivo experiments.