Open AccessThe Role of Ions on Histamine-Induced Depolarization in Isolated Guinea Pig Adipocytes
Author(s) -
Chiaki Kamei,
Yukihiko Sugimoto
Publication year - 1994
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.66.465
Subject(s) - depolarization , ouabain , histamine , chemistry , tetrodotoxin , guinea pig , choline , biophysics , tris , endocrinology , medicine , pharmacology , biochemistry , biology , sodium , organic chemistry
Effects of ions on histamine (Hi)-induced depolarization were studied in guinea pig adipocytes. Depolarization induced by Hi in guinea pig adipocytes was decreased by removal of K+ from the medium or pretreatment with ouabain at concentrations that showed no significant effect themselves. The decrease in membrane potentials induced by Hi was also abolished potently by replacement of Na+ by choline or pretreatment with tetrodotoxin at a concentration that caused no significant action alone. Pretreatment with monensin at a concentration lower than that eliciting the action resulted in a potentiation of Hi-induced depolarization. The depolarization induced by Hi was not affected by the presence of Ca2+ in the medium or pretreatment of the cells by diltiazem.