Open AccessExpression and Functional Characterization of a Rat Sulfotransferase (ST1A1) cDNA for Sulfations of Phenolic Substrates in COS-1 Cells
Author(s) -
Shogo Ozawa,
Kiyoshi Nagata,
Da-Wei Gong,
Yasushi Yamazoe,
Ryuichi Kato
Publication year - 1993
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.61.153
Subject(s) - complementary dna , sulfotransferase , sulfation , northern blot , blot , cdna library , biology , microbiology and biotechnology , southern blot , messenger rna , dehydroepiandrosterone , transferase , endocrinology , biochemistry , medicine , chemistry , gene , enzyme , hormone , androgen
A cDNA (PST-1) isolated from a rat liver cDNA library was expressed in COS-1 cells and found to encode a form of arylsulfotransferase (termed ST1A1), which sulfated p-nitrophenol, alpha-naphthol, minoxidil, beta-estradiol and dopamine, but not dehydroepiandrosterone and cortisol. ST1A1 showed a mobility identical with a major immunodetectable sulfotransferase contained in the livers of male and female rats in Western blots. In addition, ST1A1 mRNA was detected in the liver and extrahepatic tissues by Northern blots.