Expression and Functional Characterization of a Rat Sulfotransferase (ST1A1) cDNA for Sulfations of Phenolic Substrates in COS-1 Cells | Zendy

Shogo Ozawa | Zendy; Kiyoshi Nagata | Zendy; DaWei Gong | Zendy; Yasushi Yamazoe | Zendy; Ryuichi Kato | Zendy

Open Access

Expression and Functional Characterization of a Rat Sulfotransferase (ST1A1) cDNA for Sulfations of Phenolic Substrates in COS-1 Cells

Author(s) -

Shogo Ozawa,

Kiyoshi Nagata,

DaWei Gong,

Yasushi Yamazoe,

Ryuichi Kato

Publication year - 1993

Publication title -

the japanese journal of pharmacology

Language(s) - English

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.61.153

Subject(s) - complementary dna , sulfotransferase , sulfation , northern blot , blot , cdna library , biology , microbiology and biotechnology , southern blot , messenger rna , dehydroepiandrosterone , transferase , endocrinology , biochemistry , medicine , chemistry , gene , enzyme , hormone , androgen

A cDNA (PST-1) isolated from a rat liver cDNA library was expressed in COS-1 cells and found to encode a form of arylsulfotransferase (termed ST1A1), which sulfated p-nitrophenol, alpha-naphthol, minoxidil, beta-estradiol and dopamine, but not dehydroepiandrosterone and cortisol. ST1A1 showed a mobility identical with a major immunodetectable sulfotransferase contained in the livers of male and female rats in Western blots. In addition, ST1A1 mRNA was detected in the liver and extrahepatic tissues by Northern blots.

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