Open AccessNew Ulcerative Colitis Model Induced by Sulfhydryl Blockers in Rats and the Effects of Antiinflammatory Drugs on the Colitis
Author(s) -
Hiroshi Satoh,
Fumihiko Sato,
Kenji Takami,
Sándor Szabó
Publication year - 1997
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.60.299
Subject(s) - ulcerative colitis , medicine , colitis , gastroenterology , iodoacetamide , inflammation , myeloperoxidase , pathogenesis , diarrhea , pharmacology , pathology , chemistry , disease , enzyme , cysteine , biochemistry
Phenylephrine displacement curves for the specific binding of [3H]prazosin in the membrane fraction prepared from rabbit thoracic aorta showed high- and low-affinity sites with slope factors significantly less than unity. The irreversible alpha 1B-antagonist phenoxybenzamine shifted the binding sites to single high affinity sites with a slope factor close to unity in the presence of the metabolically stable GTP analog GTP gamma-S. These results indicate that phenoxybenzamine may have affected selectively the low affinity site to phenylephrine in the presence of GTP gamma-S.