Open AccessTryptophan Inhibits the [3H] Glutamate Uptake into Xenopus Oocytes Injected with Rat Brain mRNA
Author(s) -
Michihisa Tohda,
Hisashi Urushihara,
Yasuyuki Nomura
Publication year - 1992
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.59.477
Subject(s) - xenopus , messenger rna , glutamate receptor , chemistry , tryptophan , medicine , endocrinology , biology , amino acid , microbiology and biotechnology , biochemistry , gene , receptor
We characterized the glutamate (Glu) uptake in Xenopus oocytes injected with rat brain mRNA. The Glu uptake into oocytes was higher in mRNA-injected oocytes than in vehicle-injected ones. Na+ omission or addition of tryptophan inhibited the uptake in mRNA-injected oocytes, although it did not affect that in vehicle-injected oocytes. These results suggest that Glu transporters with a tryptophan sensitivity different from that of Glu transporters in native oocytes are expressed after injection of rat brain mRNA.