Open AccessEthacrynic Acid-Sensitive and ATP-Dependent Cl- Transport in the Rat Kidney
Author(s) -
Yukiko Kunugi,
Yasuko Hiraoka,
Yoshikatsu Hashimoto,
Takashi Taniguchi,
Chiyoko Inagaki
Publication year - 1991
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.57.167
Subject(s) - ouabain , gtp' , atpase , chemistry , vesicle , membrane , biochemistry , furosemide , nucleotide , biophysics , enzyme , sodium , biology , organic chemistry , gene
Ouabain- and furosemide-insensitive and ATP-dependent Cl- uptake was demonstrated in rat renal membrane vesicles. Such a Cl- uptake activity was prominent in cortical plasma membrane fractions with high activities of Cl(-)-ATPase and Na+, K(+)-ATPase. The membrane vesicles accumulated Cl- in an osmotically reactive manner with the following sequence of nucleotide specificity: ATP greater than ITP greater than UTP greater than GTP greater than CTP., beta, gamma-Methylene ATP, ADP and AMP had no effect. ATP-dependent Cl- uptake was markedly inhibited by a Cl(-)-ATPase inhibitor, ethacrynic acid (0.3 mM), but not affected by an H(+)-ATPase inhibitor, N,N'-dicyclohexylcarbodiimide (0.1 mM). These findings suggest that an ethacrynic acid-sensitive and ATP-driven