Open AccessEffects of Genistein on ATP Induced DNA Synthesis and Intracellular Alkalinization in Chang Liver Cells
Author(s) -
K.H. Sit,
Kim-Ping Wong,
BoonHuat Bay
Publication year - 1991
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.57.109
Subject(s) - genistein , intracellular , dna synthesis , extracellular , adenosine triphosphate , cell growth , biology , stimulation , dna , cell , biochemistry , microbiology and biotechnology , endocrinology
Extracellular ATP (adenosine 5'-triphosphate) produces a dose-dependent elevation of DNA synthesis concomitantly with raised intracellular pH (pHi) in quiescent monolayer Chang liver cells. Genistein produces a dose-dependent suppression of ATP-induced DNA synthesis without affecting cell viability (dead cell index approximately 5%) or pHi elevation. The results suggest that (a) mitogenesis and pHi are not imperative correlates and (b) genistein is effective in inhibiting DNA synthesis whether due to growth factor or "non-growth factor" stimulation.