Open AccessDetection of G Proteins in Bovine Brain Clathrin Coated Vesicles with Common Alpha and Beta Subunits Antibodies.
Author(s) -
Walter I. Silva
Publication year - 1991
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.56.97
Subject(s) - clathrin , western blot , protein subunit , beta (programming language) , vesicle , microbiology and biotechnology , blot , clathrin adaptor proteins , antibody , alpha (finance) , biology , chemistry , biochemistry , gene , genetics , medicine , construct validity , nursing , membrane , computer science , patient satisfaction , programming language
Clathrin coated vesicles (CV) from bovine brain were analyzed via Western blots for the presence of the alpha and beta subunits of guanine nucleotide regulatory proteins. The results with the common alpha antibody GA/1 revealed the presence of apparently undissociated G-protein subunits migrating at approximately 80 kDa. The predominant band was of about 39-41 kDa, with minor labeling observed in the 41-52 kDa range. Western blot analysis for G beta subunit(s) revealed the presence of a single band of about 35-36 kDa.