Open AccessBovine brain coated vesicles contain guanine nucleotide regulatory proteins.
Author(s) -
Kayoko Moroi,
Tetsurō Kuga,
Ken Kadota
Publication year - 1991
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.55.399
Subject(s) - guanosine , guanine , gtp' , nucleotide , biochemistry , guanosine triphosphate , guanosine diphosphate , vesicle , gtp binding protein regulators , chemistry , g protein , nad+ kinase , protein subunit , microbiology and biotechnology , biology , enzyme , receptor , gene , membrane
Binding of [3H]guanosine triphosphate (GTP) with a high affinity was found to be present in the coated vesicle fraction prepared from bovine cerebral cortex. The binding was saturable and displaced by 1 microM of GTP, guanosine diphosphate and guanosine 5'-(3-O-thio)triphosphate. Incubation of the vesicles with islet-activating protein and [32P]NAD resulted in ADP-ribosylation of a 39,000-41,000-dalton polypeptide. Antibodies to the alpha-subunit of stimulatory guanine nucleotide regulatory proteins (G-proteins) immunoblotted 52,000- and 45,000-dalton polypeptides. The results indicated that stimulatory and inhibitory G-proteins are contained in a fraction of the bovine brain coated vesicles.