Open AccessHepatic level of mRNA in relation to polymorphism and strain difference in expression of cytochrome P-450g.
Author(s) -
Kiyoshi Nagata,
Masaaki Miyata,
M. Shimada,
Yasushi Yamazoe,
Ryuichi Kato
Publication year - 1989
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.51.585
Subject(s) - messenger rna , medicine , biology , endocrinology , protein expression , translation (biology) , microbiology and biotechnology , protein biosynthesis , gene , biochemistry
Hepatic level of mRNA for P-450g was determined in male and female rats of Sprague-Dawley, ACI and Fischer strains using a specific oligonucleotide probe. The mRNA level in female Sprague-Dawley and Fischer rats was undetectable in accordance with a very low or undetectable level of P-450g protein. The mRNA was detected in all male ACI rats, in which high levels of P-450g protein were detected. In Fischer male rats, mRNA for P-450g was clearly detected in the liver, but P-450g protein was not detected. Moreover, the mRNA was detected in the liver of all male Sprague-Dawley rats, irrespective of the expressed level or absence of P-450g protein. As a most plausible explanation of the observed discordance in the levels of P-450g mRNA and protein, translation of unstable protein for microheterogeneous P-450g is discussed.