Open AccessInfluence of Bay K 8644 on aequorin-loaded human platelets.
Author(s) -
Norio Ogawa,
Hiroshi Ono
Publication year - 1989
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.51.306
Subject(s) - aequorin , platelet , thrombin , cytoplasm , chemistry , biophysics , bay , platelet aggregation , calcium , biochemistry , medicine , biology , civil engineering , engineering , organic chemistry
In aequorin-loaded human platelets, Bay k 8644 (1-100 microM) had no effect on the resting level of cytoplasmic Ca2+ and did not induce aggregation by itself. Though the rise of cytoplasmic Ca2+ and platelet aggregation induced by thrombin (0.1 U/ml) also was not inhibited by 1-10 microM of Bay k 8644, at concentration of 3-10 microM, it dose-dependently reduced those induced by collagen (5 micrograms/ml). The present study shows that human platelets do not have dihydropyridine sensitive Ca channels.