Open AccessGlutathione S-transferases and chloroform toxicity in streptozotocin-induced diabetic rats.
Author(s) -
Yoko Aniya,
Yoshihiko Ojiri,
Ryuji Sunagawa,
Keiji Murakami,
Guan Zhen-zhong,
Goro Mimura,
Matao Sakanashi
Publication year - 1989
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.50.263
Subject(s) - streptozotocin , glutathione , toxicity , microsome , phenobarbital , glutathione s transferase , diabetes mellitus , chemistry , transferase , endocrinology , medicine , pharmacology , biochemistry , biology , in vitro , enzyme
The glutathione S-transferase activity in liver and kidney cytosol was significantly decreased in short term diabetes induced with streptozotocin, whereas no decrease in the transferase was observed in phenobarbital-treated diabetic rats. Toxicity of chloroform was potentiated in streptozotocin- or phenobarbital-treated rats. The decrease in liver cytosolic and microsomal glutathione S-transferase activity was observed in long term diabetic rats, and only microsomal transferase activity was restored by insulin treatment. There was no release of glutathione S-transferases into the serum in the diabetic rats, and the transferases were not inhibited by streptozotocin in vitro. These results showed that glutathione S-transferase activity decreased during diabetes, and this decrease may contribute to altering drug metabolism and toxicity in diabetes.