Open AccessUse of cultured renal epithelial cells for the study of cisplatin toxicity.
Author(s) -
Nobuyuki Fukuishi,
Munekazu Gemba
Publication year - 1989
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.50.247
Subject(s) - nephrotoxicity , cisplatin , toxicity , in vitro , cell culture , kidney , chemistry , pharmacology , enzyme , drug , biochemistry , biology , medicine , chemotherapy , endocrinology , organic chemistry , genetics
The nephrotoxicity of cisplatin was evaluated in an in vitro system with an established cell line of normal rat kidney, NRK-52E. Leakage of enzymes from the cells into the culture medium increased when they were exposed to 1 microM of cisplatin for 72 hr. The level of lipid peroxides increased in the cells after 48 hr of exposure to cisplatin; the increase was more rapid than the enzyme leakage. This culture system can be used to evaluate drug-induced nephrotoxicity and its mechanism.