Open AccessEffect of diethylnitrosamine on monoamine oxidase in rat liver.
Author(s) -
Toshio Obata,
Hiroto Yamashita,
Toru Egashira,
Yasumitsu Yamanaka
Publication year - 1989
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.49.151
Subject(s) - monoamine oxidase , chemistry , carcinogen , monoamine oxidase a , monoamine oxidase b , enzyme , biochemistry , 2 acetylaminofluorene , pharmacology , biology , microsome
The effect of diethylnitrosamine (DEN), a well-known experimental carcinogen, toward MAO-A and MAO-B activity of rat liver was investigated. The oxidations of both beta-PEA (MAO-B) and 5-HT (MAO-A) were inhibited by DEN. The K1 values of DEN in the inhibition of rat liver MAO-A and MAO-B activity were determined. The kinetic data show that DEN is a competitive, MAO-B selective inhibitor and its inhibitory effect on MAO-B is about 4-fold more potent than that on MAO-A. DEN might change the proportions of the multiple forms of MAO activity in tumor cells.