Open AccessEffect of heptaminol AMP amidate, a new nucleotide derivative, on in vitro humoral immunity.
Author(s) -
A. Saha,
Koichi Ueno,
Shigeru Ohmori,
Takashi Igarashi,
Haruo Kitagawa
Publication year - 1988
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.47.63
Subject(s) - lipopolysaccharide , in vitro , antigen , immune system , spleen , microbiology and biotechnology , intracellular , humoral immunity , chemistry , nucleotide , theophylline , antibody , biology , biochemistry , immunology , pharmacology , gene
Heptaminol AMP amidate (HAA), a newly developed derivative of 5'-AMP, was found to potentiate the in vitro primary humoral immune response against T cell-dependent antigen, sheep red blood cells, when HAA was present in the early phase of spleen cell culture. Such a potentiating effect was not found against T cell-independent antigens such as lipopolysaccharide (LPS), trinitrophenylated (TNP)-LPS and TNP-Ficoll. The pattern of HAA-mediated immunopotentiation was similar to that of dibutyryl cyclic AMP. When HAA was added to the culture simultaneously with theophylline and imidazole, the immunopotentiating effect of HAA was further augmented and suppressed, respectively. The present results suggested that HAA-mediated immunopotentiation might be in some way related to the intracellular level of cyclic nucleotides in the early phase of culture.