Open AccessEffect of alismol on adrenergic mechanism in isolated rabbit ear artery.
Author(s) -
Hisashi Matsuda,
Johji Yamahara,
Gorô Kobayashi,
Hajimè Fujimura,
Kazuyoshi Kurahashi,
Motohatsu Fujiwara
Publication year - 1988
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.46.331
Subject(s) - stimulation , human ear , nad+ kinase , adrenergic , contraction (grammar) , medicine , endocrinology , artery , chemistry , biology , pharmacology , biochemistry , enzyme , receptor , physics , acoustics
Effects of alismol, a sesquiterpenoid isolated from the rhyzome of Alisma orientale, on adrenergic mechanisms were examined in the isolated rabbit ear artery. Alismol (10(-6) to 10(-4) M) inhibited the contraction of isolated rabbit ear artery by electrical stimulation of the perivascular nerves. The inhibition was concentration-dependent; at a concentration of 10(-4) M, the inhibition was 90% (n = 8). Treatment with 10(-4) M alismol inhibited the increase in 3H-noradrenaline (3H-NAd) release induced by electrical stimulation by 63 +/- 6%. Alismol at 10(-4) M did not affect the neuronal uptake of 3H-NAd in the artery. Alismol at 10(-4) M slightly inhibited contractions induced by exogenously administered NAd. These results demonstrate that alismol inhibits the adrenergic neuro-effector mechanisms in rabbit ear artery, and they suggest that alismol acts primarily on nerve terminals and inhibits their responses to electrical stimulation by interfering with NAd release.