Open AccessInhibitory effects of forskolin on vascular smooth muscle of rabbit aorta.
Author(s) -
Asaki Abe,
Hideaki Karaki
Publication year - 1988
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.46.293
Subject(s) - forskolin , contraction (grammar) , medicine , endocrinology , norepinephrine , chemistry , caffeine , aorta , vascular smooth muscle , biology , smooth muscle , stimulation , dopamine
Effects of forskolin on the contractions in rabbit aorta were examined. The sustained contraction induced by 10(-6) M norepinephrine was inhibited by 10(-8)-10(-5) M forskolin in a concentration-dependent manner. In the high K+-depolarized and verapamil-treated aorta, the norepinephrine-induced sustained contraction was similarly inhibited by forskolin. However, forskolin showed only a slight inhibitory effect on the sustained contraction induced by 65.4 mM KCl. Forskolin inhibited the increase in Ca2+ influx due to norepinephrine, but not that due to high K+. In a Ca2+-free solution, 10(-6) M norepinephrine induced a transient contraction which is due to Ca2+ release from the store site. This contraction was inhibited by 3 X 10(-7)-10(-5) M forskolin. However, caffeine-induced transient contraction was not inhibited by 10(-5) M forskolin. 45Ca2+ in a cellular site was released by 10(-6) M norepinephrine or 10 mM caffeine. Forskolin inhibited the Ca2+ release induced by norepinephrine, but not that by caffeine. Forskolin increased the tissue cAMP content in resting, 10(-6) M norepinephrine-treated or 65.4 mM K+-treated aorta. It is concluded that forskolin inhibits the norepinephrine-induced sustained contraction by relatively selectively inhibiting the receptor-linked Ca2+ channel, and it inhibits the norepinephrine-induced transient contraction by inhibiting Ca2+ release from the cellular store.