Open AccessEffect of concanavalin A on 5'-nucleotidase activity of rabbit blood platelets.
Author(s) -
Hiroaki Nishio,
Keiko Takeshita,
Kimiko Okugawa,
Tomio Segawa
Publication year - 1987
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.43.230
Subject(s) - concanavalin a , platelet , 5' nucleotidase , adenosine , adenosine deaminase , nucleotidase , antagonist , chemistry , medicine , biochemistry , endocrinology , microbiology and biotechnology , biology , receptor , in vitro
Ecto-5'-nucleotidase (ecto-5'-NU) of platelets was enhanced by concanavalin A (Con A). This effect of Con A was antagonized by alpha-methyl-D-mannose, a specific antagonist of Con A binding to glycoprotein. Coformycin, an adenosine deaminase inhibitor, did not change the effect of Con A on the ecto-5'-NU. Uptake of adenosine by platelets was not affected by Con A. It was suggested that the ecto-5'-NU of platelet might be a direct and primary site of action of Con A.