Open AccessL-Methionine Enhances the Contractile Responses of Rat Uterine Smooth Muscle to Acetylcholine and High KCl
Author(s) -
Seiji Ichida
Publication year - 1986
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.40.469
Subject(s) - acetylcholine , ovariectomized rat , endocrinology , medicine , egta , stimulation , contraction (grammar) , smooth muscle , methionine , chemistry , muscle contraction , calcium , biology , estrogen , biochemistry , amino acid
The contractile responses of isolated uterine smooth muscle from estradiol-treated ovariectomized rats to acetylcholine and high KCl in Ca-depleted modified Locke-Ringer (without CaCl2 and with 0.1 mM EGTA) solution on addition of CaCl2 (final concentration, 5 mM) are used as an indicator of Ca influx through the Ca channel. L-Methionine significantly enhanced these responses in the absence and presence of 3-deazaadenosine plus homocysteine thiolactone, blockers of methylation. These findings suggest that methylations of some components in isolated uterine smooth muscle seem to be important in stimulation-contraction coupling in the muscle.