Open AccessDivalent Cation Dependent Conformations of Brain Calmodulin Detected by 1H NMR
Author(s) -
Den’etsu Sutoo,
Kayo Akiyama,
Naoyuki Fujii,
Kazuhiro Matsushita
Publication year - 1986
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.40.169
Subject(s) - divalent , calmodulin , chemistry , molar ratio , proton nmr , crystallography , calcium , stereochemistry , nuclear magnetic resonance spectroscopy , biochemistry , organic chemistry , catalysis
The effects of the Ca2+, Mg2+, Zn2+, Cd2+, Hg2+ and Mn2+ on the conformation of calmodulin (CaM) have been tested by using 400 MHz 1H NMR. In the aromatic region of the spectrum of CaM with a one molar ratio of cation per CaM, Zn2+, Cd2+, Hg2+ and Mn2+ induced spectral changes which were very similar to those seen for Ca2+. However, the Mg2+-induced change was different. These results are consistent with our pharmacological findings on divalent cations.