Open AccessHydrocortisone-Evoked Molecular Conversion of Alkaline Phosphatase in Suckling Rat Small Intestine
Author(s) -
Yosuke Tojyo,
Kooichi Saida,
Aya Suzuki
Publication year - 1985
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.39.105
Subject(s) - alkaline phosphatase , hydrocortisone , medicine , endocrinology , duodenum , polyacrylamide gel electrophoresis , small intestine , enzyme , chemistry , phosphatase , biology , biochemistry
Suckling rats were injected with hydrocortisone at 12 and 13 days after birth and were sacrificed for the experiment at 15 days. Alkaline phosphatase in the duodenum was detected as three activity bands on SDS-polyacrylamide gel electrophoresis, while in control rats, the enzyme showed a single band. The electrophoretic pattern in hydrocortisone-treated rats was similar to that observed in adult rats. This result supports the view that the maturation of intestinal alkaline phosphatase is primarily regulated by glucocorticoids.