Open AccessInability of Rat Anaphylatoxin to Induce Histamine Release in Rats
Author(s) -
Satoshi Konno,
Susumu Tsurufuji
Publication year - 1985
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.38.185
Subject(s) - anaphylatoxin , histamine , zymosan , chemistry , vascular permeability , inflammation , endocrinology , pharmacology , immunology , medicine , complement system , biology , biochemistry , immune system , in vitro
The role of rat anaphylatoxin in histamine release and increased vascular permeability during the first thirty minute period in zymosan-air-pouch inflammation, an experimental model of inflammation induced by zymosan in an air-pouch prepared on the back of rats, was investigated. Complement depletion by cobra venom factor did not affect the histamine release nor the increased vascular permeability in the inflammation of this type. In spite of apparent anaphylatoxin activity, zymosan activated serum (ZAS) failed to cause any significant release of histamine when infused in the air-pouch on the back. Anaphylatoxin purified from rat serum activated with zymosan in the presence of an inhibitor (epsilon-aminocaproic acid) of anaphylatoxin inactivator gave a single band in both polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. The molecular weight estimated by SDS-PAGE was approx. 7,000. The purified rat anaphylatoxin failed to induce histamine release nor increased vascular permeability even at 50 micrograms/ml, although it caused contraction of guinea pig ileum at 0.8 micrograms/ml. These results suggest that rat anaphylatoxin does not participate in histamine release and increased vascular permeability in the zymosan-air-pouch inflammation.