Open AccessAn experimental model of nephritis induced by calf serum injection in mice.
Author(s) -
Masahiro Tsuchimoto,
Keiji Komoriya,
Tamotsu Koyama,
Kei Hosoda,
Toru Takeshita,
Tatsuyuki Naruchi,
Eiji Matsuura,
Ikuo Yamamoto
Publication year - 1984
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.36.223
Subject(s) - leukocytosis , nephritis , karyorrhexis , glomerular basement membrane , proteinuria , medicine , glomerulonephritis , cyclophosphamide , endocrinology , pathology , chemistry , kidney , chemotherapy , apoptosis , biochemistry , programmed cell death
Acute glomerulonephritis characterized by proteinuria, hypoalbuminemia and leukocytosis was induced in mice by repeated intraperitoneal injections of calf serum (1 ml/mouse X 10). In mice treated with calf serum, hypercellularity, karyorrhexis, expansion of the mesangium and hyalinosis in the glomeruli were observed by light microscopy. Furthermore, circulating immune complexes were detected in the serum, and deposits of mouse IgG and C3 on the basement membranes of the glomeruli were demonstrated immunohistochemically. Oral administration of cyclophosphamide or 6-mercaptopurine at a dose of 20 mg/kg/day significantly suppressed the development of this nephritis. Dexamethasone (0.5 mg/kg/day) caused moderate inhibition of the nephritic changes. These results suggest that this experimental model may be useful for evaluation of anti-nephritic drugs.