Potentiation of EPN-induced Inhibition of Liver Microsomal Carboxylesterase by Addition of Liver Cytosol from 6-Aminonicotinam de-Treated, Starved Rats | Zendy

Seiyu Sugiyama | Zendy; Tetsuo Satoh | Zendy; Koichi Ueno | Zendy; Takashi Igarashi | Zendy; Haruo Kitagawa | Zendy

Open Access

Potentiation of EPN-induced Inhibition of Liver Microsomal Carboxylesterase by Addition of Liver Cytosol from 6-Aminonicotinam de-Treated, Starved Rats

Author(s) -

Seiyu Sugiyama,

Tetsuo Satoh,

Koichi Ueno,

Takashi Igarashi,

Haruo Kitagawa

Publication year - 1984

Publication title -

the japanese journal of pharmacology

Language(s) - English

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.35.433

Subject(s) - carboxylesterase , cytosol , long term potentiation , microsome , nicotinamide , chemistry , cytochrome , biochemistry , endocrinology , medicine , enzyme , pharmacology , biology , receptor

Addition of liver cytosol from 16 hr-starved rats treated with 6-aminonicotinamide to rat liver microsomes caused potentiation of the anti-carboxylesterase action of ethyl-p-nitrophenyl phenylphosphonothioate (EPN). This was not found when liver cytosol from non-pretreated rats after 16 hr-starvation was used. This potentiation of EPN-induced inhibition of carboxylesterase may be, at least in part, explained by the fact that treatment of rats with 6-aminonicotinamide resulted in a significant increase in NADPH level in liver cytosol which, in turn, stimulated formation of an EPN oxygen analog, a potent inhibitor of carboxylesterase, through cytochrome P-450-coupled monooxygenase.

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