Effect of Neuroactive Peptides on Labeled 5-Hydroxytryptamine Release from Rat Spinal Slices in Vitro | Zendy

Huei-Yann Tsai | Zendy; Sadaaki Maeda | Zendy; Katsuya Iwatsubo | Zendy; Reizo Inoki | Zendy

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Effect of Neuroactive Peptides on Labeled 5-Hydroxytryptamine Release from Rat Spinal Slices in Vitro

Author(s) -

Huei-Yann Tsai,

Sadaaki Maeda,

Katsuya Iwatsubo,

Reizo Inoki

Publication year - 1984

Publication title -

the japanese journal of pharmacology

Language(s) - English

Resource type - Journals

eISSN - 1347-3506

pISSN - 0021-5198

DOI - 10.1254/jjp.35.403

Subject(s) - neurotensin , somatostatin , chemistry , substance p , medicine , neuropeptide , serotonergic , endocrinology , neurotransmitter , enkephalin , baclofen , serotonin , pharmacology , biochemistry , biology , agonist , opioid , receptor

Effects of neuroactive peptides on the release of labeled 5-hydroxytryptamine (5-HT) from preloaded rat spinal cord slices were investigated. The 5-HT release was significantly stimulated by somatostatin (10-50 microM) and substance P (10-50 microM), but not by neurotensin (50 microM), beta-endorphin (30 microM) and methionine-enkephalin (met-enk) (100 microM). Somatostatin-stimulated 5-HT release was markedly inhibited by gamma-aminobutyric acid (GABA) (30 microM), but not by baclofen (30 microM) and met-enk (100 microM). Substance P-stimulated 5-HT release was strongly inhibited by GABA (30 microM) and baclofen (30 microM), but not by met-enk (100 microM). High concentrations (20 mM) of potassium also stimulated 5-HT release. The high potassium-stimulated 5-HT release was not affected by GABA (30 microM) and met-enk (100 microM). These results suggested further evidence on the important role of somatostatin and substance P as modulators of serotonergic neurones.

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