Open AccessAphidicolin: A specific inhibitor of DNA synthesis in synchronous mastocytoma P-815 cells.
Author(s) -
Atsushi Ichikawa,
Manabu Negishi,
Kazuyoshi Tomita,
Susumu Ikegami
Publication year - 1980
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.30.301
Subject(s) - aphidicolin , mastocytoma , dna synthesis , microbiology and biotechnology , dna , cell cycle , dna polymerase , biology , cytotoxicity , biochemistry , cell , chemistry , in vitro
The influence of aphidicolin on cell multiplication and DNA synthesis was examined using synchronous mouse mastocytoma P-815 cells. Aphidicolin was cytotoxic specifically to the cells of the S phase of the cell cycle. This cytotoxicity was reversed by appropriately washing the drug-treated cells, but not by the addition of deoxyribonucleosides. Aphidicolin, a potent inhibitor of DNA synthesis, selectively inhibited the activity of partially purified DNA polymerase alpha from the nucleus and the cytosol of mastocytoma cells, but did not affect the activity of DNA polymerase beta. Furthermore, aphidicolin had no effect on the synthesis of RNA and protein, and produced no changes in cell size at least for one generation.