Open AccessEFFECT OF ENKEPHALIN AND SUBSTANCE P ON SYMPATHETIC NERVE TRANSMISSION IN MOUSE VAS DEFERENS
Author(s) -
Tomio Segawa,
Hiroko Murakami,
Hiroshi Ogawa,
Haruaki Yajima
Publication year - 1978
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.28.13
Subject(s) - vas deferens , nalorphine , endocrinology , medicine , enkephalin , substance p , chemistry , contraction (grammar) , adrenergic , neuropeptide , (+) naloxone , receptor , opioid
Effect of methionine-, leucine-enkephalin (met-, leu-enkephalin) and substance P on the transmission in mouse vas deferens was studied. Both met- and leu-enkephalin inhibited electrically induced contraction of vas deferens at 10(-8)-10(7) M, met-enkephalin being 1.4 times more active than leu-enkephalin. Nalorphine (10(-6) M) antagonized these effects. Substance P (10(-9)-10(-7) M) had no effect on the contraction. Met- and leu-enkephalin (10(-7)-10(-5) M) decreased the high potassium induced [3H]-norepinephrine release from vas deferens, while substance P (10(-6) M) significantly increased it. Nalorphine (10(-5) M) reversed the inhibitory effect of met-enkephalin. These results indicate that these peptides modify the transmission of sympathetic nerve in mouse vas deferens.