Open AccessENHANCEMENT OF NADPH-DEPENDENT LIPID PEROXIDATION ACTIVITY BY ETHYLENE-DIAMINETETRAACETIC ACID (EDTA) IN THE PRESENCE OF FERROUS ION IN RABBIT LIVER MICROSOMES
Author(s) -
Munehiro Kitada,
Tetsuya Kamataki,
Haruo Kitagawa
Publication year - 1977
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.27.653
Subject(s) - lipid peroxidation , microsome , ferrous , microsoma , chemistry , biochemistry , incubation , in vitro , enzyme , organic chemistry
The addition of EDTA to the incubation mixture containing rabbit liver microsomes and ferrous ion resulted in 2-fold increase of lipid peroxidation activity. Such an enhancement was not observed in rat liver microsomes. The maximum lipid peroxidation activity seen in rabbit microsomes in the presence of EDTA and ferrous ion was about 80% that seen in rat liver microsomes. From these results, it is likely that low lipid peroxidation activity in rabbit liver microsomes may account for the insufficiency of an EDTA-LIKE FACTORS(S) IN RABBIT LIVER MICROSOMES.