Open AccessEFFECT OF LIPID PEROXIDATION ON PHOSPHOLIPASE A2 ACTIVITY OF RAT LIVER MITOCHONDRIA
Author(s) -
Masahide Yasuda,
Tadashi Fujita
Publication year - 1977
Publication title -
japanese journal of pharmacology/japanese journal of pharmacology
Language(s) - English
Resource type - Journals
eISSN - 1347-3506
pISSN - 0021-5198
DOI - 10.1254/jjp.27.429
Subject(s) - lipid peroxidation , mitochondrion , phospholipase a2 , ascorbic acid , ferrous , chemistry , biochemistry , phospholipase , phospholipase a , enzyme , food science , organic chemistry
Effect of lipid peroxidation on phospholipase A1 activity was examined in rat liver mitochondria. Content of lipid peroxides in rat liver mitochondria was markedly increased in the presence of ascorbic acid (0.1 mM) and ferrous ion (40 muM, as compared to the control. Phospholipase A2 activity in mitochondria was activated by approximately 60% after lipid peroxidation. Mg2+-activated ATPase activity in mitochondria was markedly stimulated by about 200% following treatment with ascorbic acid and ferrous ion. There was no difference in calcium content of mitochondria before and after lipid peroxidation.