Open AccessEffects of sodium hyaluronate on the nociceptive response of rats with experimentally induced arthritis.
Author(s) -
Shizuhiko Aihara,
Naofumi Murakami,
Ritsuko Ishii,
Kimio Kariya,
Yoko Azuma,
Kumiko Hamada,
Junji Umemoto,
Seiji Maeda
Publication year - 1992
Publication title -
nihon yakurigaku zasshi/nihon yakurigaku zasshi
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.123
H-Index - 18
eISSN - 1347-8397
pISSN - 0015-5691
DOI - 10.1254/fpj.100.359
Subject(s) - chemistry , microbiology and biotechnology , biology
Antinociceptive effects of sodium hyaluronate (Na-HA) were studied on the basis of improvement in the graded abnormal gait elicited by arthritis induced by intra-articular administration of monosodium urate crystal (MSU) to rats. One hour before MSU injection, intra-articular administration of a 1.0% solution of Na-HA with different molecular weights, ranging from 4.70 x 10(5) to 2.02 x 10(6) (HA-200), improved the score of abnormal gait in a molecular weight-dependent manner in the experimental arthritis model. Similarly, administrations of HA-200 at concentrations ranging from 0.1 to 1.0% prior to MSU treatment resulted in improvement of the score in abnormal gait in a dose-dependent manner. To elucidate the antinociceptive mechanisms of Na-HA, effects of pretreatment with Na-HA (1.0%) of different molecular weights on prostaglandin E2 (PGE2) and bradykinin (BK) releases in synovial fluid 3 hr after MSU injection were studied. Increases in PGE2 and BK concentration in the synovial fluid were depressed in a molecular weight-dependent manner by Na-HA (1.0%) pretreatment. These results indicate that Na-HA attenuates the nociceptive responses inflicted by the MSU-induced arthritis. Such an antinociceptive effect may be due to the inhibition of PGE2 and BK synthesis in the synovial joint of rats.