Open AccessEffects of a Tricaprylin Emulsion on Anti-glomerular Basement Membrane Glomerulonephritis in Rats: <i>In Vivo</i> and <i>in Silico</i> Studies
Author(s) -
Ning Liu,
Junfeng Shi,
Ying Xiao,
Misato Yasue,
Yoshiaki Takei,
Hayato Sanefuji,
Gozoh Tsujimoto,
Akira Hirasawa
Publication year - 2015
Publication title -
biological and pharmaceutical bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.635
H-Index - 117
eISSN - 1347-5215
pISSN - 0918-6158
DOI - 10.1248/bpb.b15-00124
Subject(s) - in vivo , glomerulonephritis , basement membrane , in silico , microarray analysis techniques , pharmacology , microarray , glomerular basement membrane , chemistry , kidney , medicine , biology , pathology , gene expression , gene , biochemistry , microbiology and biotechnology
Glomerulonephritis (GN) is a set of pathological conditions that result in the destruction of glomeruli and loss of renal function, commonly leading to the development of end-stage renal disease. Current pharmacotherapy is limited to immunosuppressive therapy. In the present study, we found a novel antinephritic effect of a tricaprylin emulsion in the anti-glomerular basement membrane (anti-GBM) GN rat model. We evaluated the treatment in vivo by comparing administration of the emulsion with administration of a casein kinase II (CK2) inhibitor in this rat model, and performed a gene ontology-based microarray analysis to reveal in silico the detailed mechanism of action. Our results showed that administration of the tricaprylin emulsion, or even tricaprylin alone, significantly ameliorated the anti-GBM antibody-induced renal dysfunction in these rats. We believe that tricaprylin is the key active antinephritic component of the emulsion and might be a promising drug for the effective treatment of nephritis. Moreover, with respect to microarray analysis, we developed a generally applicable and rapid method to compare gene expression profile data for multiple models of nephritis and clinical samples from a public domain microarray database.