Effect of Quinine Solutions on Intracellular Ca2+ Levels in Neuro-2a Cells-Conventional Physiological Method for the Evaluation of Bitterness- | Zendy

Tomoko Nakamura | Zendy; Takeshi Akiyoshi | Zendy; Naoko Tanaka | Zendy; Kazumasa Shinozuka | Zendy; Sumio Matzno | Zendy; Toshikatsu Nakabayashi | Zendy; Kenji Matsuyama | Zendy; Makoto Kashiwayanagi | Zendy; Takahiro Uchida | Zendy

Open Access

Effect of Quinine Solutions on Intracellular Ca2+ Levels in Neuro-2a Cells-Conventional Physiological Method for the Evaluation of Bitterness-

Author(s) -

Tomoko Nakamura,

Takeshi Akiyoshi,

Naoko Tanaka,

Kazumasa Shinozuka,

Sumio Matzno,

Toshikatsu Nakabayashi,

Kenji Matsuyama,

Makoto Kashiwayanagi,

Takahiro Uchida

Publication year - 2003

Publication title -

biological and pharmaceutical bulletin

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.635

H-Index - 117

eISSN - 1347-5215

pISSN - 0918-6158

DOI - 10.1248/bpb.26.1637

Subject(s) - quinine , thapsigargin , intracellular , nifedipine , pharmacology , channel blocker , stimulation , chemistry , phospholipase c , phospholipase , endocrinology , medicine , calcium , biochemistry , receptor , enzyme , immunology , malaria

The purpose of the present study was to examine the effect of quinine on intracellular Ca2+ ([Ca2+]i) levels in cultured neuro-2a cells, and to investigate the possibility of using [Ca2+]i levels to predict the bitterness of quinine solutions. [Ca2+]i levels in neuro-2a cells increased following stimulation by quinine in a concentration-related manner. There was a good linear correlationship between the quinine-induced increase in [Ca2+]i levels increase and the bitterness scores of the quinine solutions as assessed in human gustatory sensation tests (r2=0.918). The quinine-induced increase in [Ca2+]i levels was inhibited by thapsigargin (an inhibitor of the Ca2+ pump into intracellular stores), U73122 (an inhibitor of phospholipase C) and omega-conotoxin (an N-type Ca2+-channel blocker), but not by nifedipine (an L-type Ca2+-channel blocker).

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