Bacillus Thuringiensis δ-Endotoxin And Larval Manduca Sexta Midgut Brush-Border Membrane Vesicles Act Synergistically To Cause Very Large Increases In The Conductance Of Planar Lipid Bilayers | Zendy

Francis Martin | Zendy; Michael G. Wolfersberger | Zendy

Open Access

Bacillus Thuringiensis δ-Endotoxin And Larval Manduca Sexta Midgut Brush-Border Membrane Vesicles Act Synergistically To Cause Very Large Increases In The Conductance Of Planar Lipid Bilayers

Author(s) -

Francis Martin,

Michael G. Wolfersberger

Publication year - 1995

Publication title -

journal of experimental biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.367

H-Index - 185

eISSN - 1477-9145

pISSN - 0022-0949

DOI - 10.1242/jeb.198.1.91

Subject(s) - manduca sexta , brush border , bacillus thuringiensis , vesicle , phospholipid , midgut , conductance , lipid bilayer , membrane , bilayer , biophysics , biology , biochemistry , larva , botany , bacteria , genetics , mathematics , combinatorics

Brush-border membrane vesicles prepared from midguts of Manduca sexta larvae were incorporated into planar phospholipid bilayers. Addition of Bacillus thuringiensis delta-endotoxin to the buffered salt solutions bathing these bilayers resulted in large irreversible increases in conductance. At pH 9.6, the smallest toxin-dependent increase in bilayer conductance observed was 13 nS. Similar conductance increases were never observed in the absence of delta-endotoxin or in delta-endotoxin-treated bilayers not containing components of insect brush-border membranes.

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