Generation of Monoclonal Antibodies Detecting Specific Epitopes in Locust Antennae | Zendy

Jörg Strotmann | Zendy; Ingrid Boekhoff | Zendy; S. Göggerle | Zendy; Heinz Breer | Zendy

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Generation of Monoclonal Antibodies Detecting Specific Epitopes in Locust Antennae

Author(s) -

Jörg Strotmann,

Ingrid Boekhoff,

S. Göggerle,

Heinz Breer

Publication year - 1993

Publication title -

journal of experimental biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.367

H-Index - 185

eISSN - 1477-9145

pISSN - 0022-0949

DOI - 10.1242/jeb.175.1.45

Subject(s) - monoclonal antibody , epitope , cycloheximide , locust , biology , antigen , antibody , microbiology and biotechnology , immunohistochemistry , immunocytochemistry , immunology , protein biosynthesis , botany , endocrinology

1. Following a tissue-specific screening paradigm, monoclonal antibodies have been generated that interact with distinct subpopulations of cells in locust antennae. 2. Antigens were identified as high molecular weight components. 3. Immunoreactivity was not detectable during embryonic development, but rapidly appeared within a few hours of hatching. 4. The time course of antigen expression in antennal cells could be followed in situ as well as in vitro. 5. Expression of monoclonal antibody B14/6D2-like immunoreactivity was prevented by blocking protein synthesis with cycloheximide.

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