Oxygenational Properties and Phosphorylated Metabolic Intermediates in Blood and Erythrocytes of the Dogfish, Squalus Acanthias | Zendy

R.M.G. Wells | Zendy; Roy E. Weber | Zendy

Open Access

Oxygenational Properties and Phosphorylated Metabolic Intermediates in Blood and Erythrocytes of the Dogfish, Squalus Acanthias

Author(s) -

R.M.G. Wells,

Roy E. Weber

Publication year - 1983

Publication title -

journal of experimental biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.367

H-Index - 185

eISSN - 1477-9145

pISSN - 0022-0949

DOI - 10.1242/jeb.103.1.95

Subject(s) - squalus acanthias , incubation , spiny dogfish , chemistry , metabolite , nucleotide , oxygen , bohr effect , biochemistry , cofactor , biology , hemoglobin , enzyme , organic chemistry , oxygen–haemoglobin dissociation curve , gene

A typical whole blood O2-equilibrium curve from Squalus acanthias had a P50 of 13.2 mmHg and was slightly sigmoidal, having an n value of 1.6 at 15 degrees C, PCO2 = 2.2 mmHg (pH = 7.85). A small Bohr effect was present (phi = -0.28) together with a weak Haldane effect and no Root shift. The predominant trinucleotide, determined by thin layer chromatography, was ATP (0.44 +/- 0.13 S.D. mmol 1(-1) blood) with smaller amounts of GTP present (0.07 +/- 0.02) S.D. mmol 1(-1). Total nucleotide concentrations, determined enzymatically, were low by comparison with teleosts. Incubation of erythrocytes with or without oxygen, or in the presence of a metabolite-enriched 'cocktail' showed limited potential for phosphate cofactor regulation of blood oxygen affinity.

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