Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells | Zendy

M. Lourdes Monteiro Da Silva | Zendy; Renato A. Mortara | Zendy; Helena Cristina Barros | Zendy; Wanderley de Souza | Zendy; Luíz Rachid Trabulsi | Zendy

Open Access

Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells

Author(s) -

M. Lourdes Monteiro Da Silva,

Renato A. Mortara,

Helena Cristina Barros,

Wanderley de Souza,

Luíz Rachid Trabulsi

Publication year - 1989

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.93.3.439

Subject(s) - enteropathogenic escherichia coli , microfilament , biology , hela , actin , cytochalasin d , cytochalasin b , cytochalasin , microbiology and biotechnology , escherichia coli , electron microscope , immunofluorescence , biophysics , cytoskeleton , cell , biochemistry , immunology , optics , antibody , physics , gene

We have previously observed that enteropathogenic Escherichia coli (EPEC) adhere to HeLa cells in a localized manner, which we designated localized adherence as opposed to the diffuse pattern of adhesion. In this paper we have examined the effects of localized adherence of EPEC on the actin microfilament system of host HeLa cells. Centrifugation of bacteria onto HeLa cells improved the localized adherence and rapid rearrangements of actin filaments were detected by immunofluorescence and electron microscopy. Aggregation of microfilaments is consistently observed at the sites of localized adherence, and is abolished by cytochalasin D and low temperatures. Scanning electron microscopy indicates that these aggregates are surface microvilli entangled with attached EPEC.

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