Open AccessOsmotic gradient induces stable dome morphogenesis on extracellular matrix
Author(s) -
Sumire IshidaIshihara,
Morito Akiyama,
Kazuya Furusawa,
Isao Naguro,
Hiroki Ryuno,
Takamichi Sushida,
Seiichiro Ishihara,
Hisashi Haga
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.243865
Subject(s) - morphogenesis , extracellular matrix , biology , dome (geology) , microbiology and biotechnology , swelling , biophysics , tonicity , osmotic shock , extracellular , osmotic pressure , anatomy , materials science , biochemistry , composite material , paleontology , gene
One of the fundamental processes of morphogenesis is dome formation, but many parts of the mechanisms has been unexplored. Previous in vitro studies showed that osmotic gradient is the driving factor of the dome formation. However, these investigations were performed without extracellular matrix (ECM), which provides structural support to morphogenesis. With the use of ECM, we observed that basal hypertonic stress induced stable domes in vitro that have not been seen in previous studies. These domes developed from the ECM swelling via aquaporin water transport activity. Based on computer simulation, uneven swelling, with a positive feedback between extending cell and enhanced water transport, was a cause for dome formation. These results indicate that osmotic gradient induces dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling.