Open AccessMechanisms of malignancy in glioblastoma cells are linked to MCU upregulation and higher intracellular calcium level
Author(s) -
Xiaoyun Li,
Renza Spelat,
Anna Bartolini,
Daniela Cesselli,
Tamara Ius,
Miran Škrap,
Federica Caponnetto,
Ivana Manini,
Yili Yang,
Vincent Torre
Publication year - 2020
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.237503
Subject(s) - biology , downregulation and upregulation , gene silencing , calcium , calcium in biology , intracellular , cancer research , malignancy , microbiology and biotechnology , calcium signaling , cell growth , neuroscience , medicine , biochemistry , gene , genetics
Glioblastoma (GBM) is one of the most malignant brain tumours, and despite advances in treatment modalities, it remains largely incurable. Calcium regulation and dynamics play crucial roles in different aspects of cancer, but they have never been investigated in detail in GBM. Here, we report that spontaneous calcium waves in GBM cells cause unusual [Ca2+]i elevations (>1 µM), often propagating through tumour microtubes (TMs) connecting adjacent cells. This unusual [Ca2+]i elevation is not associated with the induction of cell death and is concomitant with overexpression of mitochondrial calcium uniporter (MCU). Here, we show that MCU silencing decreases proliferation and alters [Ca2+]i dynamics in U87 GBM cells, while MCU overexpression increases [Ca2+]i elevation in human astrocytes (HA). These results suggest that changes in the expression level of MCU, a protein involved in intracellular calcium regulation, influences GBM cell proliferation, contributing to GBM malignancy.