DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis | Zendy

Miquel Rosas-Salvans | Zendy; Jacopo Scrofani | Zendy; Aitor Modol | Zendy; Isabelle Vernos | Zendy

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DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis

Author(s) -

Miquel Rosas-Salvans,

Jacopo Scrofani,

Aitor Modol,

Isabelle Vernos

Publication year - 2019

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.227033

Subject(s) - dynactin , dynein , biology , microbiology and biotechnology , microtubule , processivity , ran , mitosis , spindle pole body , motor protein , spindle apparatus , protein subunit , kinetochore , cell division , genetics , cell , dna replication , dna , gene , chromosome

Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.

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