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Since the establishment of cell culture, common practice has been to grow adherent cells in 2D monolayers. Although cells behave completely differently when grown in these artificial conditions, the ease of 2D culturing has meant that this practice still prevails today, and adopting conditions that more closely reflect the natural microenvironment has been met with substantial inertia. The alternative, animal models that mimic natural human physiology, are less accessible, strictly regulated and require licences and expensive facilities. Although transition from 2D to 3D cell culturing is gathering momentum, there is a clear need for alternative culturing methods that more closely resemble in vivo conditions. Here we show that decellularised organs gleaned from discarded animal carcasses are ideal biomimetic scaffolds to support secondary tumour initiation in vitro. This article describes how to decellularise tissue, perform basic histochemistry and immunofluorescence procedures for cell and matrix detection; and follows cancer cell behaviour on this matrix by way of an example. As integration into the traditional work flow is easy and inexpensive we hope this article will encourage other researchers to adopt this approach.

The use of decellularised animal tissue to study disseminating cancer cells