Open AccessFunctional analyses of the CIF1-CIF2 complex in trypanosome identify the structural motifs required for cytokinesis
Author(s) -
Huiqing Hu,
Paul Majneri,
Dielan Li,
Yoshihisa Kurasawa,
A. Tai,
Gang Dong,
Ziyin Li
Publication year - 2017
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.207134
Subject(s) - cytokinesis , biology , zinc finger , coiled coil , structural motif , sequence motif , microbiology and biotechnology , phd finger , genetics , cell division , dna , biochemistry , cell , transcription factor , gene
Cytokinesis in trypanosome occurs uni-directionally along the longitudinal axis from the cell anterior towards the cell posterior and requires a trypanosome-specific CIF1-CIF2 protein complex. However, little is known about the contribution of the structural motifs in CIF1 and CIF2 to complex assembly and cytokinesis. Here, we demonstrated that the two zinc-finger motifs but not the coiled-coil motif in CIF1 are required for interaction with the EF-hand motifs in CIF2. We further showed that localization of CIF1 depends on the coiled-coil motif and the first zinc-finger motif and that localization of CIF2 depends on the EF-hand motifs. Deletion of the coiled-coil motif and mutation of either zinc-finger motifs in CIF1 disrupted cytokinesis. Further, mutation of either zinc-finger motifs in CIF1 mis-localized CIF2 to cytosol and destabilized CIF2, whereas deletion of the coiled-coil motif in CIF1 spread CIF2 over to the new FAZ and stabilized CIF2. Together, these results uncovered the requirement of the coiled-coil motif and zinc-finger motifs for CIF1 function in cytokinesis and for CIF2 localization and stability, providing structural insights into the functional interplay between the two cytokinesis regulators.