Open AccessThe armadillo protein p0071 controls KIF3 motor transport
Author(s) -
Alexander Becher,
Tim Eiseler,
Marc Porzner,
Paul Walther,
René Keil,
Susanne Bobrovich,
Meçhthild Hatzfeld,
Thomas Seufferlein
Publication year - 2017
Publication title -
journal of cell science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.384
H-Index - 278
eISSN - 1477-9137
pISSN - 0021-9533
DOI - 10.1242/jcs.200170
Subject(s) - biology , kinesin , microbiology and biotechnology , microtubule , vesicle , motor protein , secretion , transport protein , vesicular transport protein , signal transducing adaptor protein , molecular motor , dynein , biochemistry , membrane , signal transduction
We here report a novel function of the armadillo protein p0071 during KIF3/kinesin-2-mediated transport. Secretion of chromogranin A and matrix metallopeptidase 9 from pancreatic neuroendocrine tumor cells or pancreatic cancer cells, respectively, was substantially reduced following knockdown of p0071. Vesicle tracking indicated impaired directional persistence of vesicles upon p0071 depletion. This suggests a disturbed balance between plus- and minus-end directed microtubule transport in cells lacking p0071. P0071 directly interacts with the KIF3/kinesin-2 motor subunit KIF3B. Our data indicate that p0071 also interacts with the kinesin cargo adaptor protein KAP3 acting as a stabilizing linker between KIF3B and its KAP3 cargo binding entity. Thus, p0071 is required for directional vesicle movement and secretion of different KIF3-transported carriers, regulating the transport of intracellular membrane vesicles along microtubules.