Rab11a regulates Syntaxin 3 localization and microvillus assembly in enterocytes | Zendy

Byron C. Knowles | Zendy; Victoria G. Weis | Zendy; Shiyan Yu | Zendy; Joseph T. Roland | Zendy; Janice A. Williams | Zendy; Gabriela Alvarado | Zendy; Lynne A. Lapierre | Zendy; Mitchell D. Shub | Zendy; Nan Gao | Zendy; James R. Goldenring | Zendy

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Rab11a regulates Syntaxin 3 localization and microvillus assembly in enterocytes

Author(s) -

Byron C. Knowles,

Victoria G. Weis,

Shiyan Yu,

Joseph T. Roland,

Janice A. Williams,

Gabriela Alvarado,

Lynne A. Lapierre,

Mitchell D. Shub,

Nan Gao,

James R. Goldenring

Publication year - 2015

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.163303

Subject(s) - microvillus , enterocyte , microbiology and biotechnology , biology , apical membrane , cell polarity , epithelial polarity , endosome , morphogenesis , apical cell , cell , epithelium , biochemistry , small intestine , membrane , genetics , intracellular , gene

Rab11a is a key component of the apical recycling endosome that aids in the trafficking of proteins to the luminal surface in polarized epithelial cells. Utilizing conditional Rab11a-knockout specific to intestinal epithelial cells, and human colonic epithelial CaCo2-BBE cells with stable Rab11a knockdown, we examined the molecular and pathological impact of Rab11a deficiency on the establishment of apical cell polarity and microvillus morphogenesis. We demonstrate that loss of Rab11a induced alterations in enterocyte polarity, shortened microvillar length and affected the formation of microvilli along the lateral membranes. Rab11a deficiency in enterocytes altered the apical localization of syntaxin 3. These data affirm the role of Rab11a in apical membrane trafficking and the maintenance of apical microvilli in enterocytes.

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