The Tor and Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 | Zendy

M. Aihara | Zendy; Xiulian Jin | Zendy; Yusuke Kurihara | Zendy; Yutaka Yoshida | Zendy; Yuichi Matsushima | Zendy; Masahide Oku | Zendy; Yuko Hirota | Zendy; Tetsu Saigusa | Zendy; Yoshimasa Aoki | Zendy; Takeshi Uchiumi | Zendy; Tadashi Yamamoto | Zendy; Yasuyoshi Sakai | Zendy; Dongchon Kang | Zendy; Tomotake Kanki | Zendy

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The Tor and Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32

Author(s) -

M. Aihara,

Xiulian Jin,

Yusuke Kurihara,

Yutaka Yoshida,

Yuichi Matsushima,

Masahide Oku,

Yuko Hirota,

Tetsu Saigusa,

Yoshimasa Aoki,

Takeshi Uchiumi,

Tadashi Yamamoto,

Yasuyoshi Sakai,

Dongchon Kang,

Tomotake Kanki

Publication year - 2014

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.153254

Subject(s) - mitophagy , biology , microbiology and biotechnology , autophagy , signal transducing adaptor protein , mitochondrion , phosphorylation , tor signaling , autophagy related protein 13 , saccharomyces cerevisiae , cytosol , yeast , biochemistry , signal transduction , protein phosphorylation , protein kinase a , apoptosis , enzyme

When mitophagy is induced in Saccharomyces cerevisiae, the mitochondrial outer membrane protein ScAtg32 interacts with the cytosolic adaptor protein ScAtg11. ScAtg11 then delivers the mitochondria to the pre-autophagosomal structure for autophagic degradation. Despite the importance of ScAtg32 for mitophagy, the expression and functional regulation of ScAtg32 are poorly understood. In this study, we identified and characterized the ScAtg32 homolog in Pichia pastoris (PpAtg32). Interestingly, we found that PpAtg32 was barely expressed before induction of mitophagy and was rapidly expressed after induction of mitophagy by starvation. Additionally, PpAtg32 was phosphorylated when mitophagy was induced. We found that PpAtg32 expression was suppressed by Tor and the downstream PpSin3-PpRpd3 complex. Inhibition of Tor by rapamycin induced PpAtg32 expression, but could neither phosphorylate PpAtg32 nor induce mitophagy. Based on these findings, we conclude that the Tor and PpSin3-PpRpd3-related pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation.

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