A systematic RNA interference screen reveals a cell migration gene network inC. elegans | Zendy

Erin J. Cram | Zendy; Hongyu Shang | Zendy; Jean E. Schwarzbauer | Zendy

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A systematic RNA interference screen reveals a cell migration gene network inC. elegans

Author(s) -

Erin J. Cram,

Hongyu Shang,

Jean E. Schwarzbauer

Publication year - 2006

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.03274

Subject(s) - biology , rna interference , caenorhabditis elegans , cell migration , morphogenesis , microbiology and biotechnology , gene , genetic screen , cell , rna , genetics , gene regulatory network , live cell imaging , gene expression , phenotype

Cell migration is essential during embryonic development and tissue morphogenesis. During gonadogenesis in the nematode Caenorhabditis elegans, migration of the distal tip cells forms two U-shaped gonad arms. Malformation results if the distal tip cells stop prematurely or follow an aberrant path, and abnormalities are easily visualized in living nematodes. Here we describe the first comprehensive in vivo RNA interference screen for genes required for cell migration. In this non-biased screen, we systematically analyzed 16,758 RNA-interference depletion experiments by light microscopy and identified 99 genes required for distal tip cell migration. Genetic and physical interaction data connect 59 of these genes to form a cell migration gene network that defines distal tip cell migration in vivo.

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