14-3-3 and calmodulin control subcellular distribution of Kir/Gem and its regulation of cell shape and calcium channel activity | Zendy

Pascal Béguin | Zendy; Ramasubbu N. Mahalakshmi | Zendy; Kazuaki Nagashima | Zendy; Damian Hwee Kiat Cher | Zendy; Akira Takahashi | Zendy; Yuichiro Yamada | Zendy; Yutaka Seino | Zendy; Walter Hunziker | Zendy

Open Access

14-3-3 and calmodulin control subcellular distribution of Kir/Gem and its regulation of cell shape and calcium channel activity

Author(s) -

Pascal Béguin,

Ramasubbu N. Mahalakshmi,

Kazuaki Nagashima,

Damian Hwee Kiat Cher,

Akira Takahashi,

Yuichiro Yamada,

Yutaka Seino,

Walter Hunziker

Publication year - 2005

Publication title -

journal of cell science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.384

H-Index - 278

eISSN - 1477-9137

pISSN - 0021-9533

DOI - 10.1242/jcs.02321

Subject(s) - calmodulin , biology , microbiology and biotechnology , gtpase , calcium channel , subcellular localization , calmodulin binding proteins , cytoskeleton , protein subunit , calcium , cytoplasm , biochemistry , cell , chemistry , gene , enzyme , organic chemistry

Individual members of the RGK family of Ras-related GTPases, which comprise Rad, Gem/Kir, Rem and Rem2, have been implicated in important functions such as the regulation of voltage-gated calcium channel activity and remodeling of cell shape. The GTPase Kir/Gem inhibits the activity of calcium channels by interacting with the beta-subunit and also regulates cytoskeleton dynamics by inhibiting the Rho-Rho kinase pathway. In addition, Kir/Gem interacts with 14-3-3 and calmodulin, but the significance of this interaction on Kir/Gem function is poorly understood. Here, we present a comprehensive analysis of the binding of 14-3-3 and calmodulin to Kir/Gem. We show that 14-3-3, in conjunction with calmodulin, regulates the subcellular distribution of Kir/Gem between the cytoplasm and the nucleus. In addition, 14-3-3 and calmodulin binding modulate Kir/Gem-mediated cell shape remodeling and downregulation of calcium channel activity. Competition experiments show that binding of 14-3-3, calmodulin and calcium channel beta-subunits to Kir/Gem is mutually exclusive, providing a rationale for the observed regulatory effects of 14-3-3 and calmodulin on Kir/Gem localization and function.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research